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Acclimation of the marine diatom Pseudo‐nitzschia australis to different salinity conditions: effects on growth, photosynthetic activity and domoic acid content ArchiMer
Ayache, Nour; Hervé, Fabienne; Lundholm, Nina; Amzil, Zouher; Caruana, Amandine.
oxic Pseudo‐nitzschia australis strains isolated from French coastal waters were studied to investigate their capacity to adapt to different salinities. Their acclimation to different salinity conditions (10, 20, 30, 35 and 40) was studied on growth, photosynthetic capacity, cell biovolume and domoic acid (DA) content. The strains showed ability to acclimate to a salinity range from 20 to 40, with optimal growth rates between salinities 30 and 40. The highest cell biovolume was observed at the lowest salinity 20 and was associated with the lowest growth rate. Salinity did not affect the photosynthetic activity; Fv/Fm values and the pigment contents remained high with no significant difference among salinities. An enhanced production of zeaxanthin was,...
Tipo: Text Palavras-chave: Acclimation; Climate change; Domoic acid; Pseudo-nitzschia australis; Physiology; Salinity.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00585/69684/67552.pdf
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Direct evidence for toxin production by Pseudo-nitzschia plurisecta (Bacillariophyceae) and extension of its distribution area ArchiMer
Caruana, Amandine; Ayache, Nour; Raimbault, Virginie; Rétho, Michael; Hervé, Fabienne; Bilien, Gwenael; Amzil, Zouher; Chomérat, Nicolas.
We confirm an amnesic shellfish poisoning risk associated with domoic acid production by the species Pseudo-nitzschia plurisecta and determine the toxin profile, which includes three domoic acid isomers. Although the species was reported once in European waters, along the Spanish Atlantic coast, here we describe and characterize P. plurisecta collected during the first occurrence in French Atlantic waters. Three strains (IFR-PPL-15.1, 15.2 and 15.3) were isolated from French coastal water samples in June 2015. These strains were identified and characterized by microscopic observation, molecular (ITS region sequencing and phylogenetic analysis) and metabolite (toxins and pigments) analyses. The cell shape is lanceolate and the valve contains a central...
Tipo: Text Palavras-chave: Coastal monitoring; Domoic acid; Pigment composition; Pseudo-nitzschia plurisecta; Taxonomic identification; Toxic diatoms.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00505/61687/66033.pdf
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Influence of sudden salinity variation on the physiology and domoic acid production by two strains of Pseudo-nitzschia australis ArchiMer
Ayache, Nour; Hervé, Fabienne; Martin-jézéquel, Veronique; Amzil, Zouher; Caruana, Amandine.
Several coastal countries including France have experienced serious and increasing problems related to Pseudo‐nitzschia toxic blooms. These toxic blooms occur in estuarine and coastal waters potentially subject to fluctuations in salinity. In this study, we document for the first time the viability, growth, photosynthetic efficiency and toxin production of two strains of Pseudo‐nitzschia australis grown under conditions with sudden salinity changes. Following salinity variation, the two strains survived over a restricted salinity range of 30 to 35, with favorable physiological responses, as the growth, effective quantum yield and toxin content were high compared to the other conditions. In addition, high cellular quotas of domoic acid (DA) were observed at...
Tipo: Text Palavras-chave: Amnesic shellfish poisoning; Domoic acid; Harmful algae; Pseudo-nitzschia australis; Salinity stress.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00462/57385/59465.pdf
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Réponses écophysiologiques des espèces toxiques de Pseudo-nitzschia aux variations environnementales liées au changement climatique ArchiMer
Ayache, Nour.
The diatom Pseudo-nitzschia includes certain toxic species capable of producing domoic acid (DA), an amnesic neurotoxin. The proliferation of these toxic species presents many risks ranging from serious ecological and economic damage to threats to public health. Problems related to these proliferations are increasing worldwide and the need for scientist to better understand the environmental factors influencing the appearance of these blooms is more necessary than ever. In this context, several species and strains of Pseudo-nitzschia have been studied to better understand and predict their physiological responses to future climate change. In particular, the experiments carried out allowed to explore the impact of salinity variations and ocean acidification...
Tipo: Text Palavras-chave: Acide domoïque; Changement climatique; Écophysiologie; Pseudo-nitzschia; Amnesic shellfish poisoning; Climate change; Domoic acid; Ecophysiology; Pseudo-nitzschia.
Ano: 2018 URL: https://archimer.ifremer.fr/doc/00478/58969/61573.pdf
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